Table 2.
Reporter genes, activation domains, and DNA-binding domains used in yeast two-hybrid experiments
| Componenta | Description (reference) |
|---|---|
| Reporter genes | |
| E. coli lacZ* | β-Galactosidase chromogenic reporter (178) |
| S. cerevisiae MEL1 | Secretory α-galactosidase chromogenic reporter (5) |
| E. coli gusA | β-Glucuronidase chromogenic reporter (580) |
| Aspergillus oryzae lacA3 | Engineered secretory β-galactosidase chromogenic reporter (318) |
| S. cerevisiae HIS3* | Prototrophic reporter for histidine biosynthesis (673) |
| S. cerevisiae LEU2* | Prototrophic reporter for leucine biosynthesis (234) |
| S. cerevisiae URA3 | Prototrophic reporter for uracil biosynthesis (374) |
| S. cerevisiae ADE2* | Prototrophic reporter for adenine biosynthesis (299) |
| S. cerevisiae LYS2 | Prototrophic reporter for lysine biosynthesis (580) |
| Aequorea victoria GFPuv | Fluorescent reporter (107) |
| EGFP | Fluorescent reporter (613) |
| Yeast EGFP | Fluorescent reporter for flow cytometry screens (88) |
| Aureobasidium pullulans AUR1-C | Aureobasidin A resistance reporter (167) |
| Prey activation domains | |
| S. cerevisiae Gal4 AD | Gal4 activating region II (aa 768 to 881), moderate strength (178) |
| Herpes simplex virus VP16 AD | VP16 activating region (aa 413 to 490), high strength (673) |
| E. coli B42 AD | Bacterial polypeptide, weak strength (234) |
| Bait DNA-binding domains | |
| S. cerevisiae Gal4 DBD* | Binds GAL1, GAL2, and GAL7 upstream activating sequences (178) |
| E. coli repressor LexA DBD* | Binds LexA operator sequences (234) |
| H. sapiens estrogen receptor DBD | Binds estrogen receptor elements (374) |
| Bacteriophage λ repressor cI | Binds cI operator sequences (580) |
| Tet repressor | Binds Tet operator sequences (716) |
a
*, most popular options.