Fig 5.

p85 increases PERK lipid kinase activity and Akt activation. (a) Activation of Akt in response to ER stress in wild-type, p85/p85 double-knockout (DKO), or DKO MEFs reconstituted with wild-type p85α (+wt p85). Phosphorylated p-4EBP1 is indicated by the arrow. (b) In vitro lipid kinase with DAGC12 as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK) alone or in the presence of recombinant p85α wild type. The intensity of phosphorylated lipid product signal was measured using phosphor imaging screen and STORM scanner (graph shown).