Fig 7.

The C-terminal domain of hnRNP Q1 is essential for RNA binding and control of neurite morphogenesis. (A) N2A cells were transiently transfected with empty vector or expression vector encoding FLAG-hnRNP Q1 (full-length or ΔC). Cell lysates were subjected to immunoprecipitation (IP) with anti-FLAG followed by RT-PCR using specific primers. (B) Transient transfection of N2A cells with siRNA was performed as for Fig. 2A, except that FLAG-hnRNP Q1ΔC was compared with full-length hnRNP Q1. Indirect immunofluorescence was performed using anti-α-tubulin (red) and anti-GFP (green). Neurite number, total neurite length, and branch number were measured and quantified (n = 100); mean values and standard errors of the means from three independent experiments are shown in the bar graphs.