Fig 5.
ChIP analysis of candidate TAS protein binding at TAS repeats. Sg4 cells were transfected with expression vectors for HA-tagged versions of the indicated proteins, and ectopic expression of the transgenes was induced by the addition of CuSO4. At 24 h postinduction, cells were cross-linked and ChIP was performed. (A) ChIP, with primers specific for the TAS-L repeat, from cells transfected with expression vectors for the indicated tagged proteins. The two bars represent the average percentage of input DNA precipitated using a control IgG antiserum and an anti-HA polyclonal antibody, and the error bars are the standard errors from two or three independent transfections. CG8289 and Polycomb enrichments are significant (P < 0.05). (B) Same as panel A but with primers specific for the TAS-R repeat family. The Dip3 and Polycomb (P < 0.05) enrichments and the CG8289 and Row (P < 0.01) enrichments are statistically significantly different. A schematic of the respective TAS repeat structures and primers used (arrows) is below each graph. Pc, Polycomb; Telom, telomere.