Fig 2.

Importin β1 and nucleolin are required for TβRI nuclear transport. (A) TβRI IP was performed using HA antibody in cells treated and fractionated as indicated. Importin β1 (KPNB1), nucleolin (NCL), nucleophosmin (NPM), Smad2/3, and TβRI (HA tag) were detected by Western blotting. (B) MCF10A/HER2/TβRI cells were transfected with siRNAs targeting importin β1, nucleolin, or a negative-control (Ctrl) siRNA. At 48 h after transfection, cells were treated with TGF-β as indicated, fractionated, and analyzed by Western blotting. (C) IFA of cells transfected with various siRNAs and treated with TGF-β for 0.5 h. HA antibody was used to stain for HA-tagged TβRI.