Fig 3.

N1ICD^OE upregulates Pax7 and elicits coordinated changes in the expression of Notch-related genes. (A) Relative levels of N1ICD expression in myoblasts treated with adenovirus-GFP (normalized to 1) or adenovirus-Cre (n = 3). (B) Western blots showing changes in N1ICD and myogenic proteins in Rosa-N1ICD myoblasts treated with adenovirus-GFP or adenovirus-Cre. (C) Relative expression levels of myogenic genes Pax7, Myf5, MyoD, and Myogenin in myoblasts treated with adenovirus-GFP (normalized to 1) or adenovirus-Cre (n = 3). (D) Relative expression levels of Notch target genes Hes1, Hes5, Hes6, Hey1, HeyL, and Nrarpa in Rosa-N1ICD myoblasts treated with adenovirus-GFP (normalized to 1) or adenovirus-Cre (n = 3). (E) Relative expression levels of Notch receptor genes in myoblasts treated with adenovirus-GFP (normalized to 1) or adenovirus-Cre (n = 3). (F) Representative Western blot confirming the increased protein level of the Notch3 receptor in Rosa-N1ICD myoblasts treated with adenovirus-Cre. (G) NICD^OE upregulates Pax7 independently of MyoD. Primary myoblasts were isolated from MyoD^−/− mice and transfected with a RAMIC plasmid to overexpress N1ICD (+). Control (−) myoblasts were transfected with an empty plasmid.