Fig 1.

MECOM is expressed early during adipocyte differentiation. (A) Total MECOM mRNA levels (with Mds1-Evi1 and Evi1 components) at daily time points during a 3T3-L1 differentiation time course. (B) Western blot of total protein from a 3T3-L1 differentiation time course with anti-MECOM antibody that recognizes both the long (Mds1-Evi1) and short (Evi1) protein isoforms. (C and D) MECOM (total Evi1 and Mds1-Evi1) expression in fractionated adipose tissues. SVF, stromovascular fraction; Adip, mature adipocytes; Ing, inguinal; Epid, epididymal. (D) MECOM expression in NIH 3T3 fibroblasts during growth (gr), confluence (day 0), or after hormone induction (day 1). Transcripts were analyzed by real-time PCR and normalized to Tbp.