Fig 4.

Time course of 3T3-L1 differentiation following MECOM knockdown. (A to F) Real-time PCR analysis of total RNA from 3T3-L1 cells infected with lentivirus expressing control (shScr) or MECOM (shMECOM) shRNAs. gr, subconfluent growing conditions; day 0, confluent cells at time of induction with differentiation medium; days 1 to 3, time after addition of induction medium. (G) Protein expression during differentiation in 3T3-L1 cells with shRNA knockdown of MECOM. Time points are prior to differentiation (0) or days following induction (1 to 4) in cells infected with control (scramble, S) or MECOM (M) shRNAs.