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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: J Immunol. 2011 Jun 22;187(3):1150–1156. doi: 10.4049/jimmunol.1004077

Figure 5. In vivo depletion of regulatory T cells restores bulk liver T cell function following BDL.

Figure 5

B6 mice were treated with 100μg of anti-CD25 (PC61) or anti-GITR (DTA1) i.p. on days -1, 0, 5, and 7 in relation to BDL. On D8, livers were harvested and Thy1.2+ bulk T cells were isolated for confirmation of depletion or labeled with CFSE and co-cultured with allogeneic splenic DC. After 72-96 hours of co-culture, T cells were analyzed by flow cytometry to measure the (A) percentage of FoxP3+ cells among CD4 T cells remaining after depletion and (B) proliferation by CFSE dissolution. For proliferation data, the bar graphs show percentage of cells that divided. On the histograms, solid lines represent T cells stimulated with DC and the dashed lines unstimulated T cells. (C) IFNγ in supernatants from the anti-CD25 depletion experiment was analyzed by cytometric bead array confirm enhanced T cell function following Treg depletion. Data are representative of 3 independent experiments.