Figure 5.

Colocalization of the two YlMdh2p isoforms with cytosolic or peroxisomal forms of the RedStar2 protein. eYFP-tagged peroxisomal YlMdh2p (eYFP-MDHp), cytosolic YlMdh2p (eYFP-MDHc) or wild-type YlMdh2p (eYFP-MDHwt) were co-expressed with either the peroxisomal (redstar2p) or cytosolic (redstar2c) forms of the RedStar2 protein. For each strain, both proteins (MDH and RedStar2) were visualized simultaneously by fluorescence microscopy. Cells were imaged after 12h of growth in YNBE 2% OA using differential interference contrast (DIC) for eYFP fluorescence (eYFP, green) and for RedStar2 fluorescence (redstar2, red). eYFP and RedStar2 images were merged (right panel). Yellow colour indicates overlapping fluorescence and evidences colocalization.