Table 3.
Number of reads specific for the short or long intron 2 of YlMDH2 obtained by RNA-seq analysis under various growth conditions
| Carbon source | Illumina Solexa sequencing technology and read length | Total reads | Reads mapping short intron 2 | Reads mapping long intron 2 | Ratio short/long |
|---|---|---|---|---|---|
| Triolein | GAIIX single reads 36 nt | 19 589 043 | 94 (57%) | 71 (43%) | 1.33 |
| Tributyrin | GAIIX single reads 36 nt | 15 996 762 | 298 (83%) | 59 (17%) | 5.05a |
| Glycerol | GAIIX single reads 36 nt | 22 935 285 | 308 (62%) | 186 (38%) | 1.66 |
| Alkane | GAIIX single reads 36 nt | 23 903 839 | 99 (47%) | 110 (53%) | 0.9b |
| Glucose | GAIIX single reads 36 nt | 13 022 908 | 121 (68%) | 56 (32%) | 2.16 |
| Glucose | Hiseq single reads 50 nt | 12 965 094 | 202 (67%) | 86 (33%) | 2.21 |
| Glucose | Hiseq single reads 50 nt | 10 592 928 | 203 (63%) | 116 (37%) | 1.70 |
| Oleic acid | GAIIX single reads 36 nt | 19 270 870 | 261 (61%) | 166 (39%) | 1.57 |
| Oleic acid | Hiseq paired-end 100 nt | 25 974 957 | 118 (60%) | 78 (40%) | 1.51 |
| Oleic acid | Hiseq paired-end 100 nt | 13 316 230 | 260 (67%) | 130 (33%) | 2.00 |
aThe ratio on tributyrin is statistically different from that on all other media (P < 0.0001).bThe ratio on alkane is statistically different from that on all other media, except on triolein (P = 0.0765).