Figure 5.

Effects of inhibitors on light-inducible gene expression in organelles. Inhibitors were added to cell cultures in dark conditions (1D18; time 0). Cells were then incubated for 1 h in light conditions (150 μEm⁻²s⁻¹). Total RNA was extracted from cells and used for DNA microarray analyses. Experiments were reproduced twice in biologically independent conditions and yielded essentially the same results. Final concentrations of inhibitors were as follows: cycloheximide (100 μM), DCMU (10 μM), and DBMIB (5 μM). Suitable concentrations of DCMU and DBMIB that do not affect the respiration but inhibit the cellular proliferation, cell division and light-induced oxygen evolution were determined by oxygen electrode and microscopic analysis. Cycloheximide and DBMIB inhibited the light-inducible gene expression of organelle genomes.