Figure 7. Flavopiridol inhibits the microglial/macrophage activation after SCI. (A) western blot analysis showed that flavopiridol treatment significantly reduced the SCI-induced upregulation of Iba-1 protein at 7 d post-injury. Representative immunoblots are shown in left panel of A. (B) Treatment of flavopiridol significantly reduced numbers of Iba-1⁺ microglia/macrophages in the preserved tissue at 7 d post injury, but not in the central lesion area. Right image shows lesion boundary between spared tissue and lesion cavity as indicated by GFAP staining. (C) Increases of Iba-1 and galectin 3 protein expression at 4 weeks after SCI were significantly attenuated by treatment of flavopiridol. Representative immunoblots are shown in left panel of C. *p< 0.05 vs. vehicle group. n = 3–5 in vehicle or flavopiridol groups, n = 2 in sham or sham + flavopiridol group.