Figure 6. Inhibition of p21waf1 mRNA translation. (A) Cells were incubated with 10 ng/ml SN38 for 24 h and then collected for polysome profiling. Samples were divided into four fractions. Fraction one includes the free mRNAs and monosomes, while fractions two, three and four include polysome of increasing size. The bars represent the range of two independent experiments. (B) Cells were incubated with 10 ng/ml SN38 for 24 h and analyzed for levels of 44 miRNAs shown to affect p21waf1 protein levels. (C) Cells were left untreated or incubated with 10 ng/ml SN38 for 24 h. Changes in miR-106b, miR-93 and miR-130b levels were assessed by real-time PCR.