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. 2012 Jun 11;7(6):e38917. doi: 10.1371/journal.pone.0038917

Figure 4. Simvastatin blocks ROS formation in neutrophils.

Figure 4

Isolated human neutrophils were pre-treated with Simvastatin (3 hours, 1 or 10 µM), stained with ROS-sensitive H2-DCFDA and then activated with fMLP. In addition, neutrophils were pre-treated with simvastatin as indicated and fluorescence was measured by flow cytometry FACS Canto (Becton Dickinson, San Jose, CA) every ten minutes following fMLP stimulation. n = 6 for each group; repetition = 4. Statistical significance was tested using one way ANOVA with Newman-Keuls Multiple Comparison test. * indicates significant difference in comparison to the control group at each different time point.