Figure 4. Effect of KCl on vesicular hyaluronan synthesis.

Inside-out vesicles were prepared from human skin fibroblasts and incubated for 3 hours with radioactive substrate for hyaluronan synthesis with the salts indicated in the absence (open bars) and presence 10 mM ATP (solid bars). A. Radioactivity incorporated into [¹⁴C] hyaluronan was determined from triplicate samples. *p<0.01 (ANOVA test). B. Size determination of vesicles incubated for 2 hours in the absence (- - -) and presence (___) of substrates for hyaluronan synthesis.