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. 2012 Jun 11;7(6):e38777. doi: 10.1371/journal.pone.0038777

Figure 5. GEP100-dependent Rac1 activation requires ERK activity.

Figure 5

(A) EGF-mediated activation of Rac1 requires ERK. HepG2 cells were treated with 10 µM U0126 for 60 min prior to EGF treatment (10 ng/mL) for 30 min, and then subjected to Rac1 activation analysis. (B) EGF-mediated ERK activation is Rac1 independent. Cells transfected with Rac1-T17N were stimulated with EGF for 15 min, and then subjected to ERK analysis. *: P<0.05, referring to the difference between cells treated with and those without EGF. #: P<0.05 (t-test), referring to the difference between cells treated with EGF plus U0126 relative to cells treated with EGF alone.