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. 2012 Jun 11;7(6):e38834. doi: 10.1371/journal.pone.0038834

Figure 1. Timeline of dietary studies.

Figure 1

All mice were weaned on Chow diet at 4 weeks of age. In the ‘prevention’ and ‘bioavailability’ studies, 3-month-old mice were switched from Chow to cHF, cHF+ω3TG and cHF+ω3PL diets for a period of 2 or 9 weeks, respectively, as indicated. In the ‘reversal study’, mice were fed cHF diet for a period of 4 month, followed by 9-week-feeding using cHF, cHF+ω3TG and cHF+ω3PL diets. In the ‘bioavailability’ study, cHF+ω3TG and cHF+ω3PL diets with several different concentrations of DHA and EPA were used (see Methods and Figure 2A,B), while in the ‘prevention’ and ‘reversal’ studies these diets were matched for their DHA/EPA content (i.e. 30 g DHA/EPA per kg diet), except for the ‘prevention’ study, in which a subgroup of mice was also treated using cHF+ω3PL diet containing 10 g DHA/EPA per kg diet. See Methods for further details. IPGTT, intraperitoneal (i.p.) glucose tolerance tests.