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. 2012 Jun 11;7(6):e38592. doi: 10.1371/journal.pone.0038592

Figure 6. RyhB has no significant affect on VirB-dependent phenotypes in S. dysenteriae expressing Altered virB.

Figure 6

A. Congo red binding by wild-type and Altered virB in S. dysenteriae in the presence (pryhB) and absence (pQE2) of increased production of RyhB. All strains were cultured in the presence of 200 µM IPTG to induce expression of ryhB from pryhB. B. Quantification of plaques formed by S. dysenteriae expressing wild-type (dark bar) or Altered virB (light bar) in the presence of ryhB expressed from the inducible plasmid promoter of pryhB and that measured in each strain carrying the vector control. All strains were cultured in the presence of 200 µM IPTG to induce expression of ryhB from pryhB. Data represents the average of four independent experiments. *represents a significant difference from the number of plaques formed by the strain carrying the pQE vector control (p≤0.01).