Primers
|
virB2-for |
TCCAATCGCGTCAGAACTTAACT
|
Real-time primer-Taqman |
virB2-rev |
CCTTTAATATTGGTAGTGTAGAACTAAGAGATTC
|
Real-time primer-Taqman |
sodB1-for |
CTGGAAAAGTCGCTGAAGCTATC
|
Real-time primer-Taqman |
sodB1-rev |
CGCTTTGAAATCGGCAAAG
|
Real-time primer-Taqman |
rrsA-for |
CACGATTACTAGCGATTCCGACTT
|
Real-time primer-Taqman |
rrsA-rev |
CGTCGTAGTCCGGATTGGA
|
Real-time primer-Taqman |
virB 3′-for |
GCGCGAAAGTCACTCGTC
|
Real-time primer-SYBR |
virB 3′-rev |
GAGATTCATTAGCCTTTTCAAGTCC
|
Real-time primer-SYBR |
virB 5′-for |
AACAGAAGAATCATTAGCCGATA
|
Real-time primer-SYBR |
virB 5′-rev |
TACGAGTGCCATCCAGAA
|
Real-time primer-SYBR |
sodB-for |
TCCGCTGCTGACCGTTGATG
|
Real-time primer-SYBR |
sodB-rev |
CGCCCAGAAGTGCTCCAGATAG
|
Real-time primer-SYBR |
rrsA-for |
AACGTCAATGAGCAAAGGTATTAA
|
Real-time primer-SYBR |
rrsA-rev |
TACGGGAGGCAGCAGTGG
|
Real-time primer-SYBR |
MBG608 |
TCCAGAATTCAGTTGATTTGAC
|
Construction of pSRG12 |
MBG607 |
ACAAAAGCTTGAACATTGGGTCATATTACA
|
Construction of pSRG12 |
virB5 |
GCTCTAGAGCAAAAGATGTAACCACTTCCC
|
Construction of altered virB
|
virB6 |
GCTCTAGAGAACGAAGTCTGTTAATGGTGG
|
Construction of altered virB
|
virB12 |
GCATTCGTGAGTTGGGCATCGGCCTTAATTTTCTGAAAGTATCAGGG
|
Construction of altered virB
|
virB13 |
TAAGGCCGATGCCCAACTCACGAATGCTATGCTC
|
Construction of altered virB
|
sodB probe for |
CTACTGGAACTGCCTGG
|
Amplification of northern blot probe |
sodB probe rev |
GTGCTCCAGATAGCCAG
|
Amplification of northern blot probe |
Probes
|
virB2 |
6FAMAGGACTTGAAAAGGCTMGBNFQ |
Real-time probe-Taqman |
sodB1 |
6FAMCCGCATCTTTTGGCMGBNFQ |
Real-time probe-Taqman |
rrsA |
6FAMATGGAGTCGAGTTGCAGMGBNFQ |
Real-time probe-Taqman |
ssvirB-sense |
TGCTCCTGCATATATTGCAGATGCTCTTCTACGAGTGCCAATTTCAATTCTACC
ATCAATCTCCCTTCCTATTACAGGGAATTGTTGTAGC
|
Northern blot |
ssvirB-antisense |
TAGCATCCGAGAACTTGGTATTGGTCTTAATTTTCTGAAAGTATCAGGGATGTCCT
ATAAAGACATAGCCAAAAAAGAGAATCTGTCTCGCGCGAAAGTC
|
Northern blot |