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. 2012 Jun 11;7(6):e38592. doi: 10.1371/journal.pone.0038592

Table 2. Primers and Probes.

Sequence Use in study
Primers
virB2-for TCCAATCGCGTCAGAACTTAACT Real-time primer-Taqman
virB2-rev CCTTTAATATTGGTAGTGTAGAACTAAGAGATTC Real-time primer-Taqman
sodB1-for CTGGAAAAGTCGCTGAAGCTATC Real-time primer-Taqman
sodB1-rev CGCTTTGAAATCGGCAAAG Real-time primer-Taqman
rrsA-for CACGATTACTAGCGATTCCGACTT Real-time primer-Taqman
rrsA-rev CGTCGTAGTCCGGATTGGA Real-time primer-Taqman
virB 3′-for GCGCGAAAGTCACTCGTC Real-time primer-SYBR
virB 3′-rev GAGATTCATTAGCCTTTTCAAGTCC Real-time primer-SYBR
virB 5′-for AACAGAAGAATCATTAGCCGATA Real-time primer-SYBR
virB 5′-rev TACGAGTGCCATCCAGAA Real-time primer-SYBR
sodB-for TCCGCTGCTGACCGTTGATG Real-time primer-SYBR
sodB-rev CGCCCAGAAGTGCTCCAGATAG Real-time primer-SYBR
rrsA-for AACGTCAATGAGCAAAGGTATTAA Real-time primer-SYBR
rrsA-rev TACGGGAGGCAGCAGTGG Real-time primer-SYBR
MBG608 TCCAGAATTCAGTTGATTTGAC Construction of pSRG12
MBG607 ACAAAAGCTTGAACATTGGGTCATATTACA Construction of pSRG12
virB5 GCTCTAGAGCAAAAGATGTAACCACTTCCC Construction of altered virB
virB6 GCTCTAGAGAACGAAGTCTGTTAATGGTGG Construction of altered virB
virB12 GCATTCGTGAGTTGGGCATCGGCCTTAATTTTCTGAAAGTATCAGGG Construction of altered virB
virB13 TAAGGCCGATGCCCAACTCACGAATGCTATGCTC Construction of altered virB
sodB probe for CTACTGGAACTGCCTGG Amplification of northern blot probe
sodB probe rev GTGCTCCAGATAGCCAG Amplification of northern blot probe
Probes
virB2 6FAMAGGACTTGAAAAGGCTMGBNFQ Real-time probe-Taqman
sodB1 6FAMCCGCATCTTTTGGCMGBNFQ Real-time probe-Taqman
rrsA 6FAMATGGAGTCGAGTTGCAGMGBNFQ Real-time probe-Taqman
ssvirB-sense TGCTCCTGCATATATTGCAGATGCTCTTCTACGAGTGCCAATTTCAATTCTACC ATCAATCTCCCTTCCTATTACAGGGAATTGTTGTAGC Northern blot
ssvirB-antisense TAGCATCCGAGAACTTGGTATTGGTCTTAATTTTCTGAAAGTATCAGGGATGTCCT ATAAAGACATAGCCAAAAAAGAGAATCTGTCTCGCGCGAAAGTC Northern blot