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. Author manuscript; available in PMC: 2013 Jul 13.
Published in final edited form as: J Mol Biol. 2012 Apr 11;420(3):141–154. doi: 10.1016/j.jmb.2012.04.007

Fig. 7.

Fig. 7

Unwinding of 16 base pair duplexes of varying stability by Dda. (a–b) Unwinding of 10 nM partial duplex substrates of varying stability by 100 nM Dda at 25°C. Data were fit using KinTek Explorer56 to Scheme 1. The rates of unwinding were 205 ± 26 bp/s, 232 ± 16 bp/s, 231 ± 10 bp/s, 234 ± 27 bp/s for unwinding of 50%, 69%, 81%, and 100% GC substrates, respectively. (c–d) Unwinding of the same substrates at 20 °C. The rate constants for unwinding were 143 ± 8 bp/s, 151 ± 9 bp/s, 148 ± 8 bp/s, 185 ± 21 s−1 for unwinding of 50%, 69%, 81%, and 100% GC substrates, respectively.