Skip to main content
. Author manuscript; available in PMC: 2013 Jul 13.
Published in final edited form as: J Mol Biol. 2012 Apr 11;420(3):141–154. doi: 10.1016/j.jmb.2012.04.007

Table 1.

The unwinding rate is insensitive to the GC content of the duplex.

% GC na L0 b(bp) kun (s−1) at 25 °C Vun (bp/s) at 25 °C kun (s−1) at 20 °C Vun (bp/s) at 20 °C
50 2 10 68.2 205 47.7 143
69 3 818 87.0 232 56.7 151
81 3 8 86.6 231 55.4 148
100 4 6 93.5 234 74.0 185
a

n is the number of steps in the unwinding mechanism (Scheme 1).

b

L0 is the number of base pairs that spontaneously melt18.