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. Author manuscript; available in PMC: 2013 Jun 29.
Published in final edited form as: Brain Res. 2012 Apr 27;1464:61–72. doi: 10.1016/j.brainres.2012.04.039

Fig. 5. Expression of neuronal and glial markers by GFP-negative PD SN AHNPs under differentiating conditions in DM2 and DM3, and a comparison of the yield of TH+ neurons among PD AHNPs from four brain regions.

Fig. 5

(A) Both DM2 and DM3 incubated cultures gave rise to neurons and astrocytes. The presence of FGF8 and SHH did not distinctly enhance TuJ1-positive, GFAP-positive and TH-positive cell numbers among the GFP-negative PD population (p>0.05). (B) PD AHNPs from SN, SVZ, HC, and CX were co-cultured with mouse GFP-ESNPs in DM3 media to induce differentiation. GFP–/TuJ1+, GFP–/TH+ and GFP–/GFAP+ cells were found in PD cell co-cultures derived from all four brain regions after 8 days of induced differentiation. SN-derived PD AHNPCs generated the lowest amount of TH+ neurons, while the highest proportion of TH+ neurons was found in CX-derived PD AHNPs (CX>HC>SVZ>SN, n=5 per region, p<0.01).