Skip to main content
. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Biochem Pharmacol. 2012 May 10;84(3):352–365. doi: 10.1016/j.bcp.2012.04.022

Figure 10.

Figure 10

Time and use dependence of mecamylamine inhibitory effects on zebrafish nAChR. Following the measurement of two control ACh control responses, cells expressing muscle-type or neuronal nAChR were switched to a bath solution containing 300 nM mecamylamine. Cells were then either stimulated with ACh and mecamylamine at 220s intervals (repeated ACh) or perfused with the mecamylamine buffer and given a single application of ACh and mecamylamine after 15 minutes. Cells were then switched back to control buffer and given 4 more stimulations with ACh alone. The data shown are peak current amplitudes for the heteromeric receptors and net charge for α7 nAChR. Each point is the average ± SEM of responses from at least four oocytes.