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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 May 13;19(6):623–627. doi: 10.1038/nsmb.2294

Figure 1.

Figure 1

Single-molecule porous vesicle encapsulation assay. (a) Schemes for the 9 bp DNA duplex and (b) DNA encapsulation; (c–l) Salt dependent dynamics for 9 bp DNA in 200 nm vesicles: single molecule time traces (c, d, e, f), and histograms from over 100 vesicles (g, h, i), at 5 mM (c, d, g), 20 mM (e, h), and 50 mM Na+ (f, i). Kd, koff, and kon vs [Na+] (j, k, l); 541 vesicles were used to calculate the rates in j, k and l. All error bars represent standard error from triplicate experiments at 23 °C. In c, green curve denotes the donor intensity and red the acceptor intensity.