Timing of dim light melatonin onset (DLMO) on the at-home vs. the in-laboratory night. Open symbols: young participants with a complaint of difficulty falling asleep and/or difficulty waking; filled symbols: older participants with a complaint of difficulty falling asleep too early and/or waking too early; dashed line: line of unity. Upper panel: DLMO was determined using a relative threshold, the value at which the level exceeded 2 standard deviations of the average of 3 baseline values. The two measures were highly correlated (r=0.68, n=20, p<0.001). Lower panel: DLMO was determined using a fixed threshold of 3 pg/mL. The two measures were highly correlated (r=0.85, n=18, p<0.0001).