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. 2012 Mar;165(5):1333–1347. doi: 10.1111/j.1476-5381.2011.01609.x

Figure 3.

Figure 3

T. cruzi invades CHO cells over-expressing ETAR or ETBR in a subtype receptor-specific manner. Transfected CHO cells over-expressing endothelin receptors (i) CHO ETAR, (ii) CHO ETBR or (iii) CHO K1 (mock control) were incubated with TCTs at parasite : cell ratio of 10:1 for 3 h in HAM's F12 medium supplemented with HAS. Where indicated, the medium was supplemented with BQ-788 (10 µM) or BQ-123 (1 µM) immediately before addition of parasites. Controls for drug vehicle involved addition of equivalent volume of HBSS or HBSS-DMSO (0.6%) to HSMC cultures treated with TCTs (not shown). After 3 h of host–parasite interaction, the monolayers were washed in HBSS, fixed with Bouin for 24 h and stained with Giemsa. The infection index represents the number of intracellular parasites per 100 cells (in triplicate) (mean ± SD). The results are representative of two independent experiments. *P < 0.05.