Figure 5.

Effects of TTX, fluorocitrate and tetanus toxin on basal and K⁺-evoked release of L-glutamate and d-serine in the mPFC. Effects of perfusion with 1 µM TTX and microinjection of 1 ng·0.3 µL⁻¹ tetanus toxin (TeNT) on the basal and K⁺-evoked release of (A) L-glutamate and (B) d-serine in the mPFC. Effects of perfusion with 1 mM fluorocitrate (FLC) on the basal and K⁺-evoked release of (C) L-glutamate and (D) d-serine in the mPFC. Grey horizontal bars: perfusion with MRS containing 1 µM TTX or 1 mM fluorocitrate; open horizontal bar: perfusion with K⁺, 100 mM (HKMRS) for 20 min. Data are means ± SEM (n = 6) of extracellular levels. The effects of microinjection of tetanus toxin and perfusion with TTX and fluorocitrate were compared using manova with Tukey's multiple comparisons (*P < 0.05; **P < 0.01 significantly different from pre-perfusion period and ^#P < 0.05, ^##P < 0.01 significantly different from control).