Figure 1.

TM-25659, a novel modulator of TAZ. (A) 3T3-L1 cells were grown to confluency and induced to differentiate under adipogenic differentiation conditions. Cells were harvested at the indicated time points, followed by oil red O staining and immunoblot analysis with antibodies against TAZ, PPARγ or C/EBPα. (B and C) TAZ stable cell line was constructed in 3T3-L1 cells by viral infection. TAZ expression was analysed by immunoblot analysis (B). The cell lines were differentiated into adipocytes for 6 days and subjected to RT and real-time PCR (C). (D) Chemical structure of TM-25659. (E) Cos-7 cells transfected with GFP-TAZ and RFP-H2B were treated with the indicated amounts of TM-25659 and quantitative nuclear intensities of TAZ were calculated using a BD Pathway HT cell-imaging platform and Bioimager software. Nuclear levels of TAZ in vehicle-treated cells was set as 100%. **P < 0.005.