Skip to main content
. 2012 Mar;165(5):1584–1594. doi: 10.1111/j.1476-5381.2011.01664.x

Figure 5.

Figure 5

Stimulatory effect of TM-25659 on osteoblast differentiation and calcium deposition. (A) C3H10T1/2 cells were induced to differentiate into osteoblasts and cultured in the presence of TM-25659 for 14 days. Calcium deposition in osteoblasts was stained with Alizarin red S (A). Dyes were dissolved in cetylpyridinium chloride, and dye absorbance was measured by spectrophotometry (OD at 405 nm) (B). Total RNA was prepared from the cells and then analysed by RT and real-time PCR. Relative expression levels of osteocalcin, ALP and osterix were calculated after normalization to the level of β-actin (C). (D) MC3T3-E1 cells were induced to differentiate into osteoblasts for 6 days in the absence or presence of TM-25659. ALP activity was determined by measuring the absorbance at 405 nm. *P < 0.05; **P < 0.005; ***P < 0.0005.