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. 2012 Mar;165(5):1595–1608. doi: 10.1111/j.1476-5381.2011.01665.x

Figure 6.

Figure 6

para-Hydroxy atorvastatin shows reduced induction of CYP protein expression. Primary human hepatocyte cultures (n = 3) were treated with 30 µM of the indicated chemicals or 0.1% DMSO only. After 48 h of treatment, total cellular protein lysates were prepared, followed by Western blot analysis of the indicated proteins. (A) Representative Western blot results from one individual culture. (B) Mean induction of CYP3A4 and CYP2B6 protein by ortho-hydroxy (o-OH-ATV) and para-hydroxy atorvastatin (p-OH-ATV) was calculated by normalizing CYP expression to β-actin and further comparing it with the extent of induction by rifampin (RIF), which was designated as 100%. Data were analysed by paired t-test. **P < 0.01, significant differences between groups as shown.