Figure 1.

Effect of CCL18 on phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and Akt. Monocytes cultured for 3 days were stimulated with 100 nm CCL18 for the indicated times. Cell lysates were prepared and immunoblotted. The blots were first probed with anti-phospho-ERK1/2 (a, b) or phospho-Akt (c), then re-probed with anti-total ERK1/2 or anti-Akt respectively. One experiment representative of four. In (a) and (c) whole cell lysates were used, whereas (b) shows cytoplasmic and nuclear fractions.