Figure 6.

Effect of adhesion molecules on CCL18 production by macrophages (MΦ): MΦ were plated on the indicated adhesion molecules on day 3 and CCL18 was detected in the supernatants by ELISA 48 hr later. In (a), 20 ng/ml interleukin-4 (IL-4) was added at the time of plating, no addition was made in (b). Statistically significant differences (Student's t test, n = 4) are indicated: *P < 0·05 or **P < 0·01. (c, d) Effect of CCL18 on integrin cell surface expression by MΦ: monocytes (day 3) were stimulated with 40 nm CCL18 for 1 hr and expression of CD11b (c) and CD11c (d) was detected by FACS (one experiment representative of two is shown).