Figure 6.

Monocytes positive to both Toll-like receptor 2 (TLR2) and TLR4 are cells in the death process. Monocytes were isolated from peripheral blood mononuclear cells by magnetic bead-labelled antibodies and stained by triple fluorescence before and after being cultured without stimulus for 24 or 48 hr at 37°. Cells were stained by AlexaFluor 488-labelled monoclonal antibody to TLR2, phycoerythrin (PE) -labelled monoclonal antibody to TLR4, and 7-aminoactinomycin-D (7-AAD) followed by flow cytometry analysis. Cells positive to 7-AAD were gated from contour plot for forward scatter versus 7-AAD staining (left panel), and analysed by TLR2 and/or TLR4 expression in healthy controls (medium panels) and patients with tuberculosis (TB) (right panel). Symbols represent the mean and standard deviation of cell subsets, n = 3.