Figure 3.
Characterization of streptozotocin (STZ) -induced diabetic acute melioidosis model. The bacterial loads in (a) liver; (b) spleen and (c) blood of STZ-induced diabetic BALB/c mice (n = 5 mice/group) at 16, 24 and 42 hr after intravenous infection with 9·36 × 103 colony-forming units (CFU)/ml Burkholderia pseudomallei. Each symbol represents one mouse; bar indicates geometric mean. Significance was determined using the Student's t-test (*P-value < 0·05; **P-value < 0·01). The control mice are not represented because no colonies were observed by plating. (d) Changes in differential leucocyte counts for both acute STZ-diabetic and acute normoglycaemic mice. Values are an average of pooled blood from three to five infected mice at a particular time-point. Data for the acute normoglycaemic infection model is adopted from Chin et al.18 (e) blood cell abnormalities.