Fig. 5.

Identification of BoLA class II restriction elements for VirB9-1, VirB9-2, and VirB10 epitopes using 293-F cells expressing bovine class II mixed isotypes. Short term T-cell lines from six animals (a) 35141, (b) 35287), and (c) 583 were cultured for three days with human embryonic kidney 293-F cells expressing CD80 and the indicated mixed isotype BoLA class II molecules that were loaded with 10 μg/ml of the indicated peptide. Results are presented as SI and T-cell responses using artificial APC pulsed with an individual peptide were compared to those of non-transfected 293-F cells incubated with the same peptide. Significant responses were determined using a Student’s one-tailed t-test, where P < 0.05, and are indicated by asterisks