Figure 5.

LPS-induced release of PGE₂ and 6-keto PGF_1α, the stable hydrolysis product of PGI₂, from human lung macrophages. Effects of the PDE4 inhibitor roflumilast, the COX inhibitor indomethacin and the COX-2 selective inhibitor NS-398. Human lung macrophages were pre-incubated with roflumilast (1 nM or 100 nM), indomethacin (1 µM), NS-398 (30 µM) or vehicle for 30 min and then stimulated with LPS (10 ng·mL⁻¹). Accumulation of PGE₂ or 6-keto PGF_1α in cell culture supernatants was measured after 24 h using commercially available EIA. Results are given as the mean ± SEM from at least five independent experiments.