Figure 1.

Peptides H2, H3, A8, H7, G4, G6, F9, F12, C6, A11 and G2 inhibit in vitro tubule formation. Co-cultures of human endothelial and interstitial cells (Angiokit) were treated in duplicates with 20 µM suramin, 5 µg·mL⁻¹ anti-Tie2 neutralizing antibody or 1 µg·mL⁻¹ of peptides. On day 14, cultures were fixed and stained for CD31, and tubule length was quantified. (A) Results are displayed as mean ± SEM of total tubule length relative to vehicle-treated cells (defined as 100%) and reflect the average obtained from four images in duplicate (total of eight measurements). *P < 0.05, **P < 0.01 significantly different from vehicle control; one-way ANOVA followed by Dunnett's post hoc test. (B) Representative images of cultures treated with vehicle control, suramin, anti-Tie2 mAb and peptide A11.