Figure 3.

Inhibition of in ovo angiogenesis in the CAM model. Tested compounds were applied on a restricted area of the CAM of fertilized eggs (n = 17–25). Peptides were tested at 0.5 and 5 nmol per egg. Fumagillin and anti-Tie2 neutralizing antibody were used as positive controls, at 5 and 0.4 µg per egg respectively. Forty-eight hours after treatment, CAMs were fixed, and total length of vessels was measured with image analysis software. Data are presented as mean ± SEM and expressed as % of vehicle control. **P < 0.01 significantly different from vehicle control; one-way ANOVA followed by Dunnett's post hoc test.