Fig. 4.

Functional consequences of the LRIT3 mutations. The T53M (T8M) mutation of LRIT3 is a known single nucleotide polymorphism (rs181200721). T53M LRIT3 was used as a control. (A and B) LRIT3 and/or FGFR1 were transfected into HEK293 cells. Transfected cells were analyzed by immunoblotting. Expression and phosphorylation status of indicated molecules were examined in the absence (A) or presence (B) of basic FGF1. A mouse monoclonal anti-LRIT3 antibody was used to visualize LRIT3. Note that all LRIT3 variants increased Golgi-modifed forms of FGFR1.