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. Author manuscript; available in PMC: 2013 Jun 19.
Published in final edited form as: Vaccine. 2012 Jun 19;30(29):4316–4322. doi: 10.1016/j.vaccine.2012.01.034

Table 2.

Titers of IgG antibody in alum control or WCV-immunized rabbit sera to live log- phase pneumococci of the indicated strain/serotype. Strains are described in Table 1 and represent a variety of clinical carriage and disease isolates as well as a library of capsule-switch variants constructed on the TIGR4 genetic background [9]. The assays were determined with and without adsorption with the cell wall polysaccharide to indicate presumed protein-specific and total antibodies, respectively. Titers are reported as reciprocal of the dilution at which OD450 0.3 was reached.

Serotype CWPS-adsorbed titer Non-adsorbed titer
Al(OH)3 control WCV-immunized Fold-difference Al(OH)3 control WCV-immunized Fold-difference
Clinical isolates
3 65 670 10 55 1000 18
4 38 1700 45 45 2300 51
4 100 2000 20 120 2900 24
4 25 1500 60 88 3200 36
6B 110 2500 23 125 3800 30
6B 25 1500 60 90 2100 23
6B 25 1600 64 92 3500 38
9V 97 1800 19 120 3800 32
9V 67 1300 19 110 3100 28
9V 54 2200 41 46 3000 65
12F 35 2100 60 39 3800 97
14 25 1400 56 90 2000 22
14 85 1800 21 90 2900 32
15B 74 3600 49 110 6600 60
23F 25 3400 140 100 5000 50
1 17 2200 130 NA NA NA
6B 58 3000 52 NA NA NA
7C 32 3300 100 NA NA NA
7F 19 3000 160 NA NA NA
14 33 2300 70 NA NA NA
18C 6 1800 300 NA NA NA
22F 31 1600 52 NA NA NA
23F 30 2300 77 NA NA NA
Isogenic TIGR4 strains
TIGR4:Capsular Type
TIGR4:1 70 2400 34 80 2900 36
TIGR4:2 88 2500 28 110 3300 30
TIGR4:3 78 3400 43 80 4000 50
TIGR4:4 85 3700 43 95 4200 44
TIGR4:5 80 2900 36 80 3400 43
TIGR4:6A 105 2900 27 110 3100 28
TIGR4:6B 100 3200 32 110 3700 34
TIGR4:7F 80 2400 30 85 2800 33
TIGR4:8 100 3000 30 120 3400 28
TIGR4:9N 100 3000 30 110 3400 31
TIGR4:9V 100 2800 28 120 3500 29
TIGR4:10A 100 2700 27 120 3800 32
TIGR4:11A 120 3100 26 130 3900 30
TIGR4:12F 70 2800 40 80 3200 40
TIGR4:14 78 3200 41 80 3400 43
TIGR4:18C 90 2800 31 95 3100 33
TIGR4:19A 90 3000 33 130 4400 34
TIGR4:19F 110 3100 28 120 4200 35
TIGR4:23F 100 2900 29 120 3400 28
TIGR4:35B 80 3000 38 80 3200 40
TIGR4: Δcapsule 100 4400 43 110 5000 45

NA – not assayed