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. Author manuscript; available in PMC: 2012 Jun 12.
Published in final edited form as: Nat Cell Biol. 2004 Jan 25;6(2):106–112. doi: 10.1038/ncb1090

Figure 5.

Figure 5

Non-polarized HeLa cells sort proteins into apical-like (PKD-independent) and basolateral-like (PKD-dependent) pathways. HeLa cells were co-transfected with PKD2-WT or PKD2-KD and either WTp75 (apical surface protein) or ΔBS-p75 (basolateral). Amounts of WTp75 or ΔBS-p75 on the cell surface were determined by surface biotinylation (see Methods). Expression of PKD2-KD selectively decreased the efficiency of transport of ΔBS-p75 to the plasma membrane but had no effect on the exocytosis of WTp75.