Table 3.
Expression of 18S rRNA related to GAPDH in three BM subsets in diseased and control samples
BM cell population | Sample identification | 18S rRNA, average CT | GAPDH, average CT | ΔCT 18S rRNA- GAPDH | ΔΔCT (ΔCT − ΔCT,C) | 18S rRNA relative to GAPDH (2−ΔΔCT) |
---|---|---|---|---|---|---|
P | (C3, C5, C6) | 20.60 ± 0.48 | 21.83 ± 0.32 | −1.24 ± 0.58 | 0.00 ± 0.58 | 1.0 (0.67–1.49) |
D2 | 19.54 ± 0.49 | 22.69 ± 0.028 | −3.155 ± 0.49 | −1.91 ± 0.49 | 3.76 (2.68–5.27) | |
D3 | 19.12 ± 0.07 | 23.20 ± 0.15 | −4.075 ± 0.17 | −2.83 ± 0.17 | 7.11 (6.32–8.00) | |
E | (C1, C3–C6) | 18.46 ± 0.87 | 21.16 ± 0.37 | −2.70 ± 0.95 | 0.00 ± 0.95 | 1.0 (0.52–1.93) |
D2 | 16.75 ± 0.00 | 20.03 ± 0.03 | −3.28 ± 0.03 | −0.58 ± 0.03 | 1.49 (1.46–1.52) | |
D3 | 17.13 ± 0.07 | 21.89 ± 0.02 | −4.76 ± 0.07 | −2.06 ± 0.07 | 4.16 (3.97–4.37) | |
M | (C1, C3–C6) | 16.38 ± 1.49 | 19.91 ± 0.99 | −3.53 ± 1.78 | 0.00 ± 1.78 | 1.0 (0.29–3.43) |
D2 | 17.30 ± 0.98 | 21.06 ± 0.63 | −3.76 ± 1.16 | −0.23 ± 1.16 | 1.17 (0.52–2.62) | |
D3 | 19.36 ± 0.64 | 22.48 ± 0.28 | −3.12 ± 0.69 | 0.39 ± 0.69 | 0.76 (0.47–0.81) |
Abbreviations: BM, bone marrow; C, control sample; CT, number of cycles; CT,C, number of control cycles; D2, diseased 2; D3, diseased 3; E, erythroid progenitor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; M, myeloid progenitor; P, multipotential progenitor.