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. 2012 May 15;3(3):e00050-12. doi: 10.1128/mBio.00050-12

FIG 1 .

FIG 1

EA increases expression of EHEC virulence genes. (A) qRT-PCR of qseC and qseE of WT EHEC strain 86-24 grown in minimal medium containing glucose (glucose minimal medium) and either NH4, EA, or NH4 plus EA to early, mid-, and late log growth phases. (B) qRT-PCR of ler of WT EHEC grown in glucose minimal medium containing either NH4, EA, or NH4 plus EA to early, mid-, and late log phases. (C) Western blots of secreted proteins from WT EHEC grown to late log in glucose minimal medium containing either NH4 or EA probed with antisera against EspA from WT EHEC grown to late log. BSA, bovine serum albumin. (D) qRT-PCR of stx2a of WT EHEC grown in glucose minimal medium containing either NH4, EA, or NH4 plus EA to early, mid-, and late log. (E) Western blots of whole-cell lysates of WT EHEC grown in glucose minimal medium containing either NH4 or EA to mid log with antisera against Stx and RpoA (loading control). qRT-PCR expression values in panels A, B, and D are presented as relative values compared to the value for WT EHEC strain 86-24 grown with NH4. Values are means plus standard deviations (error bars) for 3 independent experiments. Values that are significantly different from the value for WT EHEC strain 86-24 grown with NH4 are indicated by asterisks as follows: *, P < 0.05; **, P < 0.005; ***, P < 0.0005.