TABLE 1 .
Oligonucleotide primers used in this study
| Primera | Sequence | Function |
|---|---|---|
| eutR_LRF |
TAACTCCCTCACCCCCATTCCCG
CATCCGCTGATGCAACGTCAACGACGGCT GTGTAGGCTGGAGCTGCTTC |
Isogenic mutant construction |
| eutR_LRR |
ATCATGAAAAAGACCCGTACAGCC
AATTTGCACCATCTTTATCATGAACCCT CATATGAATATCCTCCTTAG |
Isogenic mutant construction |
| eutB_LRF |
CCGCGTCATCAGAAGAACAGTGAC
GGATCGCCCGCCCGTTTGGTCAGGCGAC GTGTAGGCTGGAGCTGCTTC |
Isogenic mutant construction |
| eutB_LRR |
CTTATGAAACTAAAGACCACATTG
TTCGGCAATGTATATCAGTTTAAGGATG CATATGAATATCCTCCTTAG |
Isogenic mutant construction |
| qseB-F | CGGTGATCCTGGATTTAACCTT | qRT-PCR |
| qseB-R | GCTGACCTTTTTCTCGCCATT | qRT-PCR |
| qseC-F | AATGGGAATACCGTGAAGACAT | qRT-PCR |
| qseC-R | CCAACCACGGGATCAATTG | qRT-PCR |
| qseE-F | ACAATCCCTGGCAATGCTTAA | qRT-PCR |
| qseE-R | GAAGCCACCAGCGAAAAGG | qRT-PCR |
| ler-F | CGACCAGGTCTGCCC | qRT-PCR |
| ler-R | GCGCGGAACTCATC | qRT-PCR |
| espA-F | TCAGAATCGCAGCCTGAAAA | qRT-PCR |
| espA-R | CGAAGGATGAGGTGGTTAAGCT | qRT-PCR |
| eae-F | GCTGGCCCTTGGTTTGATCA | qRT-PCR |
| eae-R | GCGGAGATGACTTCAGCACTT | qRT-PCR |
| stx2a-F | ACCCCACCGGGCAGTT | qRT-PCR |
| stx2a-R | GGTCAAAACGCGCCTGATA | qRT-PCR |
| qseA-F | GGCAAGGGAGATTTGTGACTAAT | qRT-PCR |
| qseA-R | GGCACCCGCCGTTAGC | qRT-PCR |
| rpoA-F | GCGCTCATCTTCTTCCGAAT | |
| rpoA-R | CGCGGTCGTGGTTATGTG | |
| eutRcompF | CTCGAGATGCCGCCACTGGTACGCTG | Construction of pMK47 |
| eutRcompR | CCCGGGCAGCTCTTTTCTGCTGGGAC | Construction of pMK47 |
a
The final letter in the primer name indicates the orientation: F, forward; R, reverse.