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Demonstration of a temperature-sensitive (Ts) growth phenotype. Mutants with Tn5-related (Tcr) insertions in genes of the σ22 stimulon were examined for growth defects compared to the parental strain, PAO1, in L broth at 45°C in a shaking water bath incubator. Among the 27 mutants examined, mutations in PA0062 or PA1324 caused a modest temperature-sensitive growth defect at 45°C. Shown is one of three experiments, all of which showed comparable results.
