Fig. 1.

Results of the expression and affinity isolation of recombinant hpTERT from E. coli cells transformed with different plasmids:  A – the hpTERT is cloned into the pCDF vector with a 6His tag at its N-terminus; B  – hpTERT is cloned into the pET33b+ vector with a 6His tag at its C-terminus; C  – hpTERT is cloned into the pET30aTEV vector with 6His- and S tags at its N-terminus. The samples of E. coli cells before and after IPTG induction of expression and the sample of elution of hpTERT from Ni-NTA agarose were analyzed by denaturing PAGE electrophoresis. The zone corresponding to the mobility of the hpTERT protein in the gel is indicated by an arrow.