Fig. 3.

Recombinant hpTERT isolated from E. coli cells transformed with the pET30aTEV vector acts as a reverse transcripatase. A –  Scheme of the RNA/DNA duplex, which is used as a substrate for hpTERT; B –  Products of the reaction of hpTERT (the presence of the protein is marked by +) and different substrates (the type of the used substrate is indicated above the picture). Products of the reaction are visualized via the introduction of radioactively labeled dGTP into the reaction mixture. The zone which corresponds to the mobility of the initial DNA oligonucleotide is marked by an arrow. The primer extension is present only in the first lane; this proves that recombinant hpTERT acts as a reverse transcriptase in this model system.