Table A1. Available primers for Bartonella spp. Amplification.
Microorganism | Gene | Direction* | Primer | Sequence |
---|---|---|---|---|
Bartonella clarridgeiae | ribC | F | PBC5 | TACATAACGAGCCAATT |
B. clarridgeiae | ribC | R | PBC15 | TAGCTTTAGAACAATATGGT |
B. henselae | ribC | F | PBH-L1 | GATATCGGTTGTGTTGAAGA |
B. henselae | ribC | R | PBH-R1 | AATAAAAGGTATAAAACGCT |
B. bacilliformis | ribC | F | PBH-L1 | GATATCGGTTGTGTTGAAGA |
B. bacilliformis | ribC | R | PBB-R1 | AAAGGCGCTAACTGTTC |
B. quintana | ribC | F | PBH-L1 | GATATCGGTTGTGTTGAAGA |
B. quintana | ribC | R | PBQ-R1 | AAAGGGCGTGAATTTTG |
All Bartonella | ribC | F | PBH3 | CCAAGTGCTACATAACCATC |
All Bartonella | ribC | R | PBH4 | CGGGTTGTTATTGCTCTTAC |
All (except B. bacilliformis) | 16s-23s spacer | F | 16SF | AGAGGCAGGCAACCACGGTA |
All (except B. bacilliformis) | 16s-23s spacer | R | 23S1 | GCCAAGGCATCCACC |
All Bartonella | 16s-23s spacer | F | QHVE1 | TTCAGATGATGATCCCAA |
All Bartonella | 16s-23s spacer | R | QHVE2 | TTGGGATCATCATCTGAA |
All Bartonella | 16s-23s spacer | F | QHVE3 | GATATATTCAGACATGTT |
All Bartonella | 16s-23s spacer | R | QHVE4 | AACATGTCTGAATATATC |
B. bacilliformis | 16s-23s spacer | F | BABF | CTGGATCACCTCCTTTCTAA |
B. bacilliformis | 16s-23s spacer | R | BABR | ATGCCCTTAAGACACTTGAT |
B. quintana | 16s-23s spacer | F | BQF | CTCCACCATTTTAGGTCATC |
B. quintana | 16s-23s spacer | R | BQR | GGTTTTGAGAATTCCCTTGC |
All Bartonella | 16s-23s spacer | F | 16s1 | (C/T)CTTCGTTTCTCTTTCTTCA |
All Bartonella | 16s-23s spacer | R | 16s2 | GGATAAACCGGAAAACCTTC |
All Bartonella | 16s-23s spacer | F | 16s3 | (C/T)CTTCGTTTCTCTTTCTTCA |
All Bartonella | 16s-23s spacer | R | 16s4 | AACCAACTGAGCTACAAGCC |
All Bartonella | 16s-23s spacer | F | 16s5 | CTCTTTCTTCAGATGATGATCC |
All Bartonella | 16s-23s spacer | R | 16s6 | AACCAACTGAGCTACAAGCCCT |
All Bartonella | ribC | F | BARTON-1F | TAACCGATATTGGTTGTGTTGAAG |
All Bartonella | ribC | R | BARTON-2R | TAAAGCTAGAAAGTCTGGCAACATAACG |
All Bartonella | 16s | S | probe | ATTTGGTTGGGCACTCTAGGGG |
All Bartonella | 16s | Rp2a | ACGGCTACCTTGTTAGGACTT | |
All Bartonella | 16s | F | 357f | TACGGGAGGCAGCAG |
All Bartonella | 16s | R | 357ra | CTGCTGCCTCCCGTA |
All Bartonella | 16s | F | 536F | CAGCAGCCGCGGTAATAC |
All Bartonella | 16s | R | 536R | GTATTACCGCGGCTGCTG |
All Bartonella | 16s | F | 800F | ATTAGATACCCTGGTAG |
All Bartonella | 16s | R | 800R | CTACCAGGGTATCTAAT |
All Bartonella | 16s | F | 1050F | TGTCGTCAGCTCGTG |
All Bartonella | 16s | R | 1050R | CACGAGCTGACGACA |
All Bartonella | 35KD | F | 35KD1fa | GTCGCTAAAGGCTGATGA |
All Bartonella | 35KD | R | 35KD2ra | GACTGATATCGTGCGTGTG |
All Bartonella | 35KD | F | 35KDs1f | GGTACGACGACAGTAATTGTT |
All Bartonella | 35KD | R | 35KDs2r | GATTTAAGAGATACCAACCA |
All Bartonella | PAP | F | PAP1fa | CTTTAATGACGACTTCTGTT |
All Bartonella | PAP | R | PAP4ra | CCGAAATCTGAGTAACGGTA |
All Bartonella | PAP | R | PAP2r | CCCTAAATGTTTCAAGTTCA |
All Bartonella | PAP | F | PAP3f | GCTGACAGAGAAGACGCAA |
All Bartonella | groEL | F | BbHs233.p | CGTGAAGTTGCCTCAAAAACC |
All Bartonella | groEL | R | BbHs1630.n | AATCCATTCCGCCCATTC |
All Bartonella | rpoB | F | QVE1 | TTCAGATGATGATCCCAAGC |
All Bartonella | rpoB | R | QVE3 | AACATGTCTGAATATATCTTC |
All Bartonella | Bfp1 | ATTAATCTGCAYCGGCCAGA | ||
All Bartonella | Bfp2 | ACVGADACACGAATAACACC | ||
All Bartonella | Bfs3 | TTACAAAAATCYGTTGATAC | ||
All Bartonella | Bfs4 | GTATCAACRGATTTTTGTAA | ||
All Bartonella | ftsZ | F | BaftsZF | GCTAATCGTATTCGCGAAGAA |
All Bartonella | ftsZ | R | BaftsZR | GCTGGTATTTCCAAYTGATCT |
B. henselae, B. clarridgeiae | ftsZ | BhftsZ 1393.n | GCGAACTACGGCTTACTTGC | |
B. henselae, B. clarridgeiae | ftsZ | Bh ftsZ 1247.p | CGGTTGGAGAGCAGTTTCGTC | |
B. quintana | ftsZ | F | Bq ftsZseqF | GCACATATTCTTGATGAGAT |
B. quintana | ftsZ | R | Bq ftsZseqR | CCCCTATCATCTCATCAAG |
B. bacilliformis | ftsZ | F | Bb ftsZseqF | GCGCATGTTCTTAGTGAAAT |
B. bacilliformis | ftsZ | R | Bb ftsZseqR | CCTGTATACGTGATGCATTT |
All Bartonella | ftsZ | FTS1p | GCCTTCTCATCCTCAACTT | |
All Bartonella | ftsZ | FTS2p | CAGCCTCTTCACGATGTG | |
All Bartonella | pap31 | PAPn1 | TTCTAGGAGTTGAAACCGAT | |
All Bartonella | pap31 | PAPn2 | GAAACACCACCAGCAACATA | |
All Bartonella | pap31 | PAPns2 | GCACCAGACCATTTTTCCTT | |
All Bartonella | pap31 | PAPns1 | CAGAGAAGACGCAAAAACCT | |
All Bartonella | groEL | HSPps1 | CAGAAGTTGAAGTGAAAGAAAA | |
All Bartonella | groEL | HSPps2 | GCNGCTTCTTCACCNGCATT | |
All Bartonella | groEL | HSPps4 | GCTGGNGGTGTTGCNGTTA | |
All Bartonella | groEL | HSPps3 | GCTGTNGAAGANGGNATTGT |
*F, forward; R, reverse.