FIGURE 1.

Representative histopathology of lung lesions caused by P. brasiliensis Pb18 strain in mice immunized with P10 in presence of Freund’s complete adjuvant (CFA). Tissue samples were collected two months after i.t. challenge with the Pb18 strain. (A) Lung section from infected mouse, with a granuloma containing multiple viable fungal cells; (B) Lung section from mouse treated with CFA, showing the extensive granulomatous lesion with intense cellular infiltration and large number of multiplying fungal cells; (C) Lung section from mouse immunized with P10 admixed with CFA showing preserved alveolar structure, absence of granulomatous lesions as well as of fungal cells. All sections were amplified 10-fold and stained with hematoxylin–eosin.